Figure 2. Optimal migration of NK cells to the dLN requires autonomous expression of the chemokine receptor CXCR3.

A) Total number of NK cells in the indicated LNs of individual B6 and Cxcr3−/− mice (n=5) with mean ± SEM. B-D) Donor NK cells in dLN and ndLN at 2 dpi or uLN from B6 mice that had been co-transferred with equal numbers of CFSE labeled splenocytes from B6-CD45.1 (Cxcr3+/+) and Cxcr3−/− mice. Representative flow cytometry plots showing the gating strategy (B), frequencies of Cxcr3+/+ and Cxcr3−/− NK cells in the indicated LNs of of individual mice (n=3-5) with mean ± SEM (C), and total number of the indicated donor NK cells ± SEM in the indicated LNs of the same mice (D). All experiments were performed a minimum of two times and repeats yielded similar results.