Table 5.
Molecular and Flow Cytometry Chimerism
| Molecular Chimerism: % Donor DNA in NK-enriched MNC
| ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Blood
| ||||||||||||
| Days Post-infusion | Subject
|
|||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| 7 | 0 | 2 | 0 | 15 | 2 | 1 | 84 | 11 | 64 | 0 | 2 | 15 |
| 14 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
| 28 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
| 56 | – | 2 | 0 | – | – | – | 0 | – | – | – | – | 0 |
| 112 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| 170 | – | 0 | – | – | – | – | – | – | – | – | – | – |
|
| ||||||||||||
|
Bone Marrow
| ||||||||||||
| Days Post- infusion |
Subject
|
|||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|
| ||||||||||||
| 56 | – | 1 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | 0 |
| 112 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| 170 | – | 0 | – | – | – | – | – | – | – | – | – | – |
|
| ||||||||||||
|
Flow Cytometry Chimerism: % Donor DNA in NK-gated Population
| ||||||||||||
|
Blood
| ||||||||||||
| Days Post- infusion |
Subject
|
|||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|
| ||||||||||||
| 7 | 0.40 | 0.40 | 15.80 | ND | 66.20 | 7.03 | ||||||
| 14 | 0.09 | 0.08 | 0.00 | 0.39 | 0.00 | 2.37 | ||||||
| 28 | 0.00 | 0.03 | 0.03 | 0.00 | 0.85 | |||||||
| 56 | 0.01 | 0.02 | 0.00 | 0.03 | ||||||||
| 112 | 0.12 | |||||||||||
| 170 | 0.08 | |||||||||||
|
| ||||||||||||
|
Bone Marrow
| ||||||||||||
| Days Post- infusion |
Subject
|
|||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
|
| ||||||||||||
| 56 | 0.06 | 0.02 | 0.01 | 0.04 | ||||||||
| 112 | 0.26 | |||||||||||
| 170 | 0.58 | |||||||||||