Table 5.
Molecular and Flow Cytometry Chimerism
Molecular Chimerism: % Donor DNA in NK-enriched MNC
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Blood
| ||||||||||||
Days Post-infusion | Subject
|
|||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
7 | 0 | 2 | 0 | 15 | 2 | 1 | 84 | 11 | 64 | 0 | 2 | 15 |
14 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
28 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
56 | – | 2 | 0 | – | – | – | 0 | – | – | – | – | 0 |
112 | – | 0 | – | – | – | – | – | – | – | – | – | – |
170 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| ||||||||||||
Bone Marrow
| ||||||||||||
Days Post- infusion |
Subject
|
|||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| ||||||||||||
56 | – | 1 | 0 | – | 0 | 0 | 0 | – | 0 | 0 | – | 0 |
112 | – | 0 | – | – | – | – | – | – | – | – | – | – |
170 | – | 0 | – | – | – | – | – | – | – | – | – | – |
| ||||||||||||
Flow Cytometry Chimerism: % Donor DNA in NK-gated Population
| ||||||||||||
Blood
| ||||||||||||
Days Post- infusion |
Subject
|
|||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| ||||||||||||
7 | 0.40 | 0.40 | 15.80 | ND | 66.20 | 7.03 | ||||||
14 | 0.09 | 0.08 | 0.00 | 0.39 | 0.00 | 2.37 | ||||||
28 | 0.00 | 0.03 | 0.03 | 0.00 | 0.85 | |||||||
56 | 0.01 | 0.02 | 0.00 | 0.03 | ||||||||
112 | 0.12 | |||||||||||
170 | 0.08 | |||||||||||
| ||||||||||||
Bone Marrow
| ||||||||||||
Days Post- infusion |
Subject
|
|||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
| ||||||||||||
56 | 0.06 | 0.02 | 0.01 | 0.04 | ||||||||
112 | 0.26 | |||||||||||
170 | 0.58 |