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. 2018 Nov 12;50(11):146. doi: 10.1038/s12276-018-0173-3

Fig. 1. The expression of SIRT3 decreases in degenerated human disc tissues and increases in TBHP-treated rat NPCs.

Fig. 1

a, b Representative MR images of three different degrees of IVDD patients. a Pfirrmann grade II patient, male, 46-years old, lumbar disc herniation. b Pfirrmann grade III patient, female, 53-years old, lumbar disc herniation. c Pfirrmann grade V patient, female, 51-years old, lumbar disc herniation. Nucleus pulposus samples from different patients of ages were collected for the measurement of SIRT3, with consistency in the degree of intervertebral disc degeneration. Western blot analysis and bar diagrams of SIRT3 showed that the protein levels of SIRT3 decreased in an age-dependent manner. c Representative Western blots and bar diagram of SIRT3 levels in each human NP tissue group (Pfirrmann grades II, III, and V). d, e The effect of different concentrations of TBHP on rat NPCs. f, g The effect of different durations of treatment with TBHP on rat NPCs. All experiments were performed in duplicates, and data are reported as the mean ± SD. *P < 0.05, **P < 0.01