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. 2018 Nov 12;50(11):146. doi: 10.1038/s12276-018-0173-3

Fig. 2. The effects of SIRT3 knockdown and overexpression on apoptosis, senescence, and mitochondrial dysfunction under oxidative stress in rat NPCs.

Fig. 2

a, b Successful knockdown of SIRT3 in rat NPCs was established using LV-shSIRT3. c, d The effects of SIRT3 on the cleaved caspase-3 levels under conditions of oxidative stress. e, f The effects of SIRT3 on the p16INKa levels under conditions of oxidative stress. g, h Representative images of SA-β-gal staining of NPCs from different groups. Scale bar: 50 μM. i, j The nucleus morphology was detected using Hoechst 33342. Scale bar: 50 μM. k, l Mitochondrial membrane potential of rat NPCs was measured using MitoTracker red CMXRos. Scale bar: 50 μM. All experiments were performed in duplicate, and data are reported as the mean ± SD. *P < 0.05, **P < 0.01