Fig. 3. HKL suppresses apoptosis and senescence induced by TBHP by activating SIRT3 in rat NPCs.

a NPCs were treated with different doses of HKL for 24 h. b, c NPCs were treated with different doses of HKL as indicated. Cell lysate was harvested and analyzed for the expression of SIRT3 using Western blots. d NPCs, pretreated with different doses of HKL, were administered with TBHP. e, f SIRT3 knockdown inhibited the capacity of the anti-senescence of HKL in NPCs. Scale bar 100 μM. g, h Fluorescence microscopy of Hoechst 33342 staining for morphological assessment of apoptosis. Apoptotic nuclei were characterized by condensed chromatin and nucleus fragmentation. Scale bar, 50 μM. i–k The expressions of cleaved caspase-3 and p16INKa were detected by western blotting. All experiments were performed in duplicate, and data are reported as the mean ± SD. ^*P < 0.05, ^**P < 0.01