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. 2018 Jun 14;23(9):1851–1867. doi: 10.1038/s41380-018-0100-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Diazepam/Isoguvacine-dependent translocation of GFP-PH_PLCδ1 from the cell membrane to the cytoplasm in α₁/β₂/γ₂-HEK293 cells. a Live imaging of a Calcein blue-labelled cell (inset) showing changes in GFP-PH_PLCδ1 fluorescence intensity profile (green) prior to (left) and 5 min after the addition of Diazepam (D; 1 μM)/Isoguvacine (I; 5 μM) (right). b Quantification of fluorescence F(membrane)/F(cytoplasm) ratio of GFP-PH_PLCδ1 (green; mean ± s.e.m.; Student t-test; *p < 0.05; n = 10 cells from 2 independent experiments). c Imaging of surface GABA_AR-β₂-subunit in fixed HEK293 cells (red) expressing GFP-PH_PLCδ1 (green) in control (DMSO, left) and Diazepam (D; 1 μM)/Isoguvacine (I; 5 μM) treated samples for 1 h (right), showing superimposed fluorescence intensity profiles across the selected cells. d Quantification of fluorescence F(membrane)/F(cytoplasm) ratio of GFP-PH_PLCδ1 (green; mean ± s.e.m.; Student t-test; *p < 0.05; n = 10 cells from 2 independent experiments)