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. 2018 Nov 12;8:16671. doi: 10.1038/s41598-018-35119-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of proteomics results regarding PAI-1 on endothelial cells treated with atorvastatin (Atorva) or by DKK-1 silencing as described in the Material and methods section (n = 3). Effect on PAI-1 mRNA levels exerted by siDKK-1 (a) or atorvastatin (b). PAI-1 was evaluated at antigen levels by ELISA in cells treated with siDKK-1 (c), atorvastatin (d) or recombinant DKK-1 (e). The data are expressed as ng/ml of PAI-1. Values are the mean ± SEM of 3 individual experiments.*p < 0.05 vs siNEG or control cells.