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. 2018 Nov 6;6:334. doi: 10.3389/fped.2018.00334

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Copyright © 2018 Sawada and Inoue.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reductions in NK cells by ATG. (A) UPN605. (B) UPN612.Both were diagnosed as HMB of the NK-cell type. The absolute number of each lymphocyte subset in PB was counted on days 0, 1, and 2 before and after the administration of ATG as the initiation of RIC. Other drugs were also shown. The lymphocyte subset was analyzed by three-color flow cytometry (FCM) using fluorescein isothiocyanate-, phycoerythrin/rhodamine-, and phycoerythrin-cyanine 5-conjugated antibodies (Beckman Coulter Inc., USA). FCM was performed with EPICS XL® (Coulter Inc., USA). B, T, and NK cells were defined as CD20 + CD19 + CD45 + cells, CD3 + CD45 + cells, and CD3 - CD56 + CD45 + cells, respectively. LDEC, low-dose etoposide and cytosine arabinoside; Flu, fludarabine.