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. 2018 Nov 12;9:4757. doi: 10.1038/s41467-018-07245-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — V. cholerae complementation restores WT sensitivity to predation. In 1:1 competitions with WT, V. cholerae mutants showed similar sensitivity to B. bacteriovorus predation as in the previous Tn-seq experiments. Complemented strains showed WT levels of sensitivity to predation. CheY**, a tumble-biased strain, showed intermediate sensitivity to predation compared to WT and ∆motY strains. The average competitive indices and standard error of the mean for three to six biological replicates are shown. *P < 0.05 (ANOVA with Dunnett’s multiple comparisons test)