Fig. 4.

Rnf12 KO embryos show REX1 stabilization in embryonic and extraembryonic tissues. a Sex and genotype distribution from different matings of Rnf12-deficient mice in a C57BL/6 background. Number of breedings, number of mice per breeding and total number of mice are indicated. Note that no female embryos were born with a maternally transmitted Rnf12 deleted allele. No significant differences were observed between the number of females with a paternal mutant allele and their WT brothers (χ² test, p > 0.05). A significant slight lethality associated with the mutation in male mice compared to WT brothers was observed (χ² test, p = 1.05E−4). b Representative Z-stack projections of WT, Rnf12^−/− and Rnf12^−/y E4.5 blastocysts immunostained for REX1 (red), H3K27me3 (Xi marker, green), the trophectoderm marker CDX2 (grey) and DNA (DAPI, blue). Scale bars: 20 μm