Fig. 4. Ikaros dependence on CX-4945 promoting ARID5B expression.

a Treatment with CX-4945 induces an increase in ARID5B expression in Nalm6 and CEM cells; ***p < 0.001 compared to DMSO control. b Protein levels of ARID5B as evaluated by Western blot in the indicated cells that were incubated with different doses (10 μM, 20 μM) of CX-4945 or DMSO control (0) for 48 h. Lamin B was used for loading control. c Effect of Ikaros shRNA knockdown on the CX-4945-induced promotion of ARID5B expression. ***p < 0.01 compared to siCTL + CX4945 group; (d) CX-4945 promotes ARID5B expression in primary ALL cells; ***p < 0.001 compared to the control. Graphed data in A-D represents the mean+/– SD of triplicates representative of one of 3 independent experiments