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. 2018 Nov 12;8:16681. doi: 10.1038/s41598-018-34750-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biophysical properties of wild-type (WT) and mutant A204E hNa_v1.4 channels in HEK293 cells. (A) Representative whole-cell current traces recorded from HEK293 cells expressing wild-type (WT, black) or A204E (red) hNa_v1.4 channels in response to a test pulse of 10 ms at −10 mV from a holding potential of −120 mV are shown. (B) Normalized current/voltage relationships of WT (n = 36) and A204E (n = 28) channels. Current densities were normalized by cell capacitance. (C) Voltage dependence of activation and steady-state fast inactivation curves for WT and A204E channels were plotted and fitted with a single Boltzmann equation. (D) Effect of A204E on window current. The probability of being within this window was calculated as indicated in the materials and methods section. (E) Steady-state fast inactivation for WT and A204E channels in response to the pulse protocol shown in the inset. (F) Recovery from fast inactivation of WT and A204E channels. (G) Voltage-dependence of time constants (τ) of fast inactivation for WT (n = 9) and A204E (n = 9) channels obtained when measuring entry from −70 to −30 mV and recovery from −120 to −80 mV. The statistical differences between WT and A204E channels are shown (***P < 0.001).