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. 2018 Nov 6;5:307. doi: 10.3389/fmed.2018.00307

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Copyright © 2018 Ju, Stehbens and Haass.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Migration at the 3D Melanoma-collagen interface. (A) Melanoma spheroids (1205Lu cells) embedded in a porous fibrillar collagen I hydrogel show heterogeneous 3D migration phenotypes at the spheroid-matrix interface. Within collagen I hydrogels, melanoma cells exhibit multi-cellular streaming (red arrows), single-cell rounded migration (white arrows) and polarized mesenchymal migration (orange arrow). (B) Representative high resolution spinning disc confocal images of single melanoma cells expressing fluorescently labeled filamentous actin (mScarlet-i-Lifeact) demonstrating several 3D migration phenotypes within the same collagen I hydrogel i. Amoeboid blebbing migration ii. Intermediate blebbing-pseudopodia phenotype and iii. Mesenchymal migration.