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. 2018 Nov 12;8:16673. doi: 10.1038/s41598-018-34898-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Assessment of Toco-Photoxil on haemolysis. (a) Photographic images of RBC suspended with different concentrations (µg/ml) of Toco-Photoxil, PBS (negative control), and water (positive control) at 1 h and 24 h time period. (b) ESEM images of RBCs treated with PBS (negative control), water (positive control), and 125 µg/ml of Toco-Photoxil. Arrow heads indicate the presence of Toco-Photoxil over RBCs as confirmed by EDAX analysis. (c) Graph shows the percentage hemolysis. (d) Photographic images of supernatant used for recording the absorbance at 577 nm.