FIGURE 12.

CXCL13 mediates passive cutaneous anaphylaxis. (A) Passive cutaneous anaphylaxis was performed as described. After 1 h of Evans blue solution injection, the dye was eluted from the ear in 700 μl of formamide at 63°C. The absorbance was measured at 620 nm. Representative images from four animals of each experimental group are shown. ^∗∗∗p < 0.0005. (B) Ear tissue lysates from BALB/c mouse of each experimental group were subjected to β-hexosaminidase activity assay. ^∗∗p < 0.005; ^∗∗∗p < 0.0005. (C) Same as (B) except that western blot and immunoprecipitation were performed.