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Induction of the features of AD occurs in an MIP-2-dependent manner. Skin tissues of BALB/c mice of each experimental group were isolated at the time of sacrifice and were subjected to immunohistochemical staining by employing anti-MIP-2 antibody (2 μg/ml) and H&E staining. Toluidine blue staining was also performed to determine the number of degranulated mast cells. Closed triangle represents degranulated mast cells. ∗∗∗p < 0.0005.
