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. 2018 Nov 6;9:1175. doi: 10.3389/fphar.2018.01175

FIGURE 6.

FIGURE 6

Suppressor of cytokine signaling 1 (SOCS1) is necessary for DNCB-induced AD. (A) Skin tissue lysates of BALB/c mice of each experimental group were subjected to miRNA array analysis. (B) Shows the experimental time line to determine the effect of MIP-2 on AD (lower). Upper figure shows the effect of SOCS1 on clinical symptoms associated with AD. ∗p < 0.05; ∗∗∗p < 0.0005. (C) Tissue lysates from the mice of each experimental group were subjected to western blot or immunoprecipitation by employing the indicated antibody (2 μg/ml). Tissue lysates from BALB/c mouse injected with scr. following the induction of AD was also immunoprecipitated with isotype-matched IgG antibody (2 μg/ml). (D) Tissue lysates from the mice of each experimental group were subjected to ß-hexosaminidase activity assays (left). Sera of BALB/c mice were employed for the determination of the amount of histamine released (middle) and IgE level (right). ∗∗p < 0.005; ∗∗∗p < 0.0005.