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Apoptosis was induced in H5N1 IAV-infected pancreatic cells. (A) PAN02 and (B) PANC-1 cells were mock-treated or infected with H1N1, H5N1, or H7N2 at an MOI of 1 for 12 h. A terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay was used to measure apoptosis in PAN02 and PANC-1 cells with DNase I as a positive control. Blue showed nucleus and green showed positive TUNEL signals.
