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Pancreatic cell apoptosis was measured at specified times following IAV infection. PAN02 and PANC-1 cells were mock-treated or infected with IAV (H1N1, H5N1, or H7N2) at an MOI of 1, taxol served as a positive control. Then the cells were harvested at 6, 12, and 24 h post-infection. The total number of apoptotic cells in early stage was analyzed by flow cytometry. Asterisks indicate statistically significantly values compared with mock-treated cells (**P < 0.01, and ***P < 0.001).
