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. 2018 Nov 6;9:526. doi: 10.3389/fgene.2018.00526

FIGURE 3.

FIGURE 3

Macrophages have altered proliferative potential in lymphoid tissues in response to mesothelioma tumor. Young (n = 9) and elderly C57BL/6J mice (n = 9) were inoculated with s.c. with 5 × 105 AE17 mesothelioma tumor cells and sacrificed at day 21–23, based on the maximum allowed size (140 mm2; tumor size calculated as width × length measured using calipers). Healthy control mice were also included (n = 8 for young and n = 9 for elderly). Spleen and bone marrow samples were dissociated into single cell suspensions, stained for CD11b, F4/80, Ly6G, Siglec-F, viability dye and intracellular Ki67 and analyzed via flow cytometry. Macrophages (CD11b+F4/80+Ly6G-Siglec-F-) were measured for proliferation via Ki67 staining (shown as % positive) in the BM (example plots shown in A, pooled data in B) and spleen (example plots shown in C, pooled data in D) of young and elderly healthy and AE17 tumor bearing mice. Data is pooled from three experiments shown as mean ± SEM. ∗∗p < 0.01.