Table 1.
List of strains and plasmids used in the study.
| Strains | Description | References |
|---|---|---|
| XL-1 Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIq ZΔM15 Tn10 (Tetr)] | Stratagene, USA |
| HB-101 | F-, thi-1, hsdS20 (rB- mB), supE44, recA13, ara-14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1 | Promega, USA |
| BL-21(λDE3), plysS | F-, ompT, hsdS B (rB-mB), dcm, gal, λ(DE3), plysS, cmr | Promega, USA |
| mc2155 | M. smegmatis parental strain | A kind gift from Prof. Anil K Tyagi. |
| H37Rv | Laboratory Strain (ATCC 27294) of M. tuberculosis | ATCC |
| M. bovis BCG Danish | M. bovis BCG parental strain | A kind gift from Prof. Anil K Tyagi. |
| ΔcitE1 | citE1 mutant strain of M. tuberculosis | This study |
| ΔcitE2 | citE1 mutant strain of M. tuberculosis | This study |
| ΔcitE-DM | citE1 and citE2 double deletion strain of M. tuberculosis | This study |
| PLASMIDS | ||
| pYUB854 | Cloning vector, hygR | Bardarov et al., 2002 |
| pYUB-ΔcitE1 | pYUB854 vector carrying upstream and downstream regions of citE1, hygromycin cassette | This study |
| pYUB-ΔcitE2 | pYUB854 vector carrying upstream and downstream regions of citE2, hygromycin cassette | This study |
| pYUB-ΔcitE2 kan | pYUB854 vector carrying upstream and downstream regions of citE2, kanamycin cassette | This study |
| phAE87 | Phagemid DNA, AmpR | Bardarov et al., 2002 |
| phAE87-ΔcitE1 | Phagemid DNA carrying upstream and downstream regions of citE1, hygromycin cassette | This study |
| phAE87-ΔcitE2 | Phagemid DNA carrying upstream and downstream regions of citE2, hygromycin cassette | This study |
| phAE87-ΔcitE2 kan | Phagemid DNA carrying upstream and downstream regions of citE2, kanamycin cassette | This study |
| pET28b | E. coli T7-based expression system | Novagen |
| pET-28b-citE1 | pET28b carrying citE1 | This work |
| pET-28b-citE2 | pET28b carrying citE2 | This work |