Figure 4.

Cell intrinsic BACH2 supports T cell development and expansion. B6.Bach2^ΔT (CD45.2):B6.Bach2^fl/fl (CD45.1) (50:50) mixed bone marrow chimeric mice were generated in lethally irradiated B6.Rag1^−/− mice and engraftment measured in splenic leukocytes 12 weeks later (A). Subsequently, these mice were generated by injecting a 70:30 mix of bone marrow from B6.Bach2^ΔT and B6.Bach2^fl/fl mice into irradiated B6.Rag1^−/− mice, and the proportions of CD45.2 and CD45.1 leukocytes in the spleen were measured before P. chabaudi infection and 7 and 14 days p.i., as indicated (B). Numbers of CD4⁺ T and CD8⁺ T cells (C), as well as frequency of Th1 and Tr1 cells (D) in the spleen at days 0, 7, and 14 p.i., as indicated, were measured by flow cytometry. n = 6 mice per group per time point. Mean ± SEM, *P < 0.05, **P < 0.01, significance assessed by Mann-Whitney U-test.