Figure 4.

Ephrin-A1 reduces growth cone Tctp expression levels. (A) Nasal and temporal stage 32 retinal explants grown in vitro for 24 h were stimulated with Ephrin-A1-Fc at a concentration of 5 μg/mL, and stained for Tctp. Representative micrographs of control (clustered Fc)- and Ephrin-A1-treated RGC growth cones are shown (mean ± SEM; n = no. of growth cones analyzed; ***P < 0.0001, one-way ANOVA and Tukey’s Multiple Comparison Test). (B) Rat primary cortical neurons were incubated with rapamycin at 50 nM for up to 2 h and Tctp protein levels evaluated in whole cell extracts at various time points (mean ± SEM; n = 3 biological replicates; P = 0.9282, one-way ANOVA). Scale bar: 5 μm.