FIG 1.

HCMV IE1 deSUMOylated hPML and protected hPML from degradation by ICP0. (A, right) U-251 MG or U-251 MG-IE1 cells were mock infected or infected with WT HSV-1 (strain 17) for 24 h at an MOI of 1. The whole-cell lysates were collected for Western blotting to detect the proteins PML, IE1, ICP0, and tubulin. (Left) U-251 MG cells were mock infected (lane 1), HSV-1 infected plus MG132 treated (lane 2), or HSV-1 infected (lane 3) for 12 h. The whole-cell lysates were used for Western blotting to detect the proteins. MW, molecular weight. (B) U-251 MG or U-251 MG-IE1 cells were infected with WT HSV-1 (strain 17) for the indicated times at an MOI of 1. The whole-cell lysates were collected for Western blotting to detect the proteins PML, IE1, ICP0, and tubulin. (C) U-251 MG or U-251 MG-IE1 cells grown on coverslips were infected with WT HSV-1 (strain 17) for 16 h, and the cells were then fixed and permeabilized for IFA to detect PML, ICP0, and DAPI. The arrows show the PML in ICP0-positive cells. Bar, 5 μm.