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IFA to show that HCMV IE1 protected hPML from degradation by ICP0. (Upper) U-251 MG-IE1 cells grown on coverslips were transfected with pYFPICP0 for 24 h, and the cells were then fixed and permeabilized for IFA to detect PML, IE1, ICP0, and DAPI. The arrows show the PML in ICP0-positive cells. (Lower) The same experiments were performed in U-251 MG cells. Bar, 10 μm.
