FIG 7.

Isopycnic iodixanol gradient profiles showing density distributions of intracellular wt and mutant viral RNAs. (A) Gradients loaded with lysates from cells electroporated 72 h previously with wt (HM175/18f) (top) or the related L1,2-A mutant (bottom). Also shown in the bottom panel is the density distribution of subgenomic replicon 18f-FLuc RNA (HAV-FLuc). A unique peak of viral RNA is present in the L1,2-A gradient (1.093 g/cm³). (B) Gradients loaded with lysates from cells electroporated 9 days previously with wt (HM175/p16) (top) or the related L1-A (middle) and L2-A (bottom) mutants. Unique, low-density RNA peaks are present in gradients loaded with lysates of cells transfected with either mutant.