TABLE 1.

Impact of Tyr-to-Ala and Leu-to-Ala mutations in the VP2 late domains of HAV

Mutant	Substitution	Domain(s) affected	Capsid assemblya	eHAV release	ALIX bindingb
Y1-A	Tyr→Ala	L1	No	None
Y2-A	Tyr→Ala	L2	No	None	Noc
Y1,2-A	Tyr→Ala	L1 + L2	No	None
Y1-A/H171Nd	Tyr→Ala	L2	Yes	Yes
L1-A	Leu→Ala	L1	Yes	↓, 4-fold	Yes
L2-A	Leu→Ala	L2	Yes	↓, 2-fold
L1,2-A	Leu→Ala	L1 + L2	Yes	None	No

^aAntigen detected with K34C8 in RNA-transfected cells or release of infectious virus.

^bAnti-ALIX precipitation of encapsidated viral RNA.

^cSee the work of Feng et al. (9).

^dRevertant virus from Y2-A mutant (Fig. 2B).