FIG 1.

Binding of different categories of human anti-E MAbs to mature, immature, or mixed particles. (A) Outline of preparation of mature, mixed, and immature DENV1 (D1) virions from 293T-furin cells, 293T cells, and 293T cells in the presence of ammonium chloride, respectively. (B) WB analysis of mature, mixed, or immature DENV1 virions purified by sucrose gradient ultracentrifugation and probed with DENV-immune human serum (anti-prM/E) and rabbit serum against M-peptide (anti-M). The values on the left are molecular masses (in kilodaltons). (C) The relative prM contents of the different particles were determined by a virion ELISA, which used a murine MAb (FL0251) for capture and human MAbs (anti-E MAb DVG17.12, anti-prM MAb DVB 59.3) for detection. (D to M) Binding curves of different categories of human MAbs to mature, immature, or mixed particles based on virion-capture ELISAs, including FL-specific MAbs (D, E), DIII MAbs (F, G), EDE1 MAbs (H, I), EDE2 MAbs (J, K), and DI/IIh MAb (L, M). The relative OD (rOD) compared to the OD of DENV-immune human serum is presented. Data are the means and standard deviations for duplicates from one representative experiment of two.