FIG 8.

US27 increases NRF-1 translocation at later times during infection. (A) NuFF cells grown on glass coverslips were infected with TB40-mCherry WT or US27Δ (MOI = 0.2) and then fixed at 72 hpi and stained with anti-NRF-1 antibody and Alexa Fluor 514-conjugated secondary antibody (green) as well as DAPI (blue). mCherry (red) is a marker of infection. Bars, 20 μm. Enlarged images (right) represent the areas in the white boxes in the NRF-1 panels. These images are representative of those from one of three independent experiments. (B) Average total cellular and nuclear fluorescence was quantified for individual cells (n = 10). RFU, relative fluorescent units. Error bars represent the standard error. *, P < 0.0001 by paired Student's t test versus mock- or US27Δ-infected cells; ns, not significant.