FIG 9.
US27 activation of NRF-1 requires Gβγ and phosphatidylinositol 3-kinase (PI3K). HEK293 or 293-US27 cells were transfected with the pCignal-ARE-GFP plasmid for 24 h and then cultured overnight in the presence of the indicated chemical inhibitors, and total GFP levels were evaluated by flow cytometry. The results for a panel of inhibitors targeting G proteins (A) and other intracellular signaling proteins (B) are shown. Results are represented as the percentage of total fluorescence relative to the total fluorescence for control cells. Error bars represent the standard error. *, P < 0.001 by paired Student's t test. CTX, cholera toxin.