TABLE 2.
Primers used for generating TB40/E-mCherry-US27wt (US27wt)
| Recombinant virus (TB40/E-mCherry) | Primer direction or characteristica | Primer sequence (5′ → 3′)b |
|---|---|---|
| US28Δ-galK insertion | FOR | GGTGCGTGGACCAGACGGCGTCCATGCACCGAGGGCAGAACTGGTGCTATCcctgttgacaattaatcatcggca |
| REV | AGAGGGGCGGACACGGGGTTTGTATGAAAAGGCCGAGGTAGCGCTTTTTTAtcagcactgtcctgctcctt | |
| US28Δ | ds oligo | CAGACGGCGTCCATGCACCGAGGGCAGAACTGGTGCTATCTAAAAAAGCGCTACCTCGGCCTTTTCATACAAACCCCGTG |
| UL33Δ-galK insertion | FOR | TTCCGCCCAGACCCGCAACAACACTCCTCCGCACATCAATGACACTTGCAACcctgttgacaattaatcatcggca |
| REV | GGGGAAATGGCGACGGGTTCTGGTGCTTTCTGAATAAAGTAACAGGAAAGCtcagcactgtcctgctcctt | |
| UL33Δ | ds oligo | CCGCAACAACACTCCTCCGCACATCAATGACACTTGCAACGCTTTCCTGTTACTTTATTCAGAAAGCACCAGAACCCGTC |
| UL78Δ-galK insertion | FOR | GTCCCCGGAGAGGGTATATTCGTTCGGCGAGAGCGGGCGGCGGTGGTGGGTcctgttgacaattaatcatcggca |
| REV | TAACGTGATTTATCTGCCACTTTTCTCCCCGCTGCCGTACAGCGCCGCCGCtcagcactgtcctgctcctt | |
| UL78Δ | ds oligo | GGGTATATTCGTTCGGCGAGAGCGGGCGGCGGTGGTGGGTGCGGCGGCGCTGTACGGCAGCGGGGAGAAAAGTGGCAGAT |
a
FOR, forward primer; REV, reverse primer; ds oligo, double-stranded oligonucleotide.
b
Lowercase sequences are complementary to the galK template plasmid.