TABLE 3.
Primers for evaluating gene expression via standard PCR and qPCRa
| Target gene | Primer direction | Primer sequence (5′ → 3′) |
|---|---|---|
| CD47 | FOR | TCCACTGTCCCCACTGACTT |
| REV | TGGCAATGACGAAGGAGGTT | |
| CXCR4 | FOR | CCGTGGCAAACTGGTACTTT |
| REV | CCCTTGGAGTGTGACAGCTT | |
| GAPDH | FOR | ACCACAGTCCATGCCATCAC |
| REV | TCCACCACCCTGTTGCTGTA | |
| IE1 | FOR | GGTCACTAGTGACGCTTGTATGATGACCAGTACCGA |
| REV | GATAGTCGCGGGTACAGGGGACTCT | |
| NRF-1 | FOR | AGCAAAAGCAGAGGGTTTCA |
| REV | CTGTGTTTGCGTTTGCTGAT | |
| NRF-2 | FOR | GAGAGCCCAGTCTTCATTGC |
| REV | TGCTCAATGTCCTGTTGCAT | |
| US27 | FOR | GACGGACAAGCTTCGATGACCACCT |
| REV | CGACGAGCTGCAGTTACAACAGAAA | |
| β-Actin | FOR | ATTAAGGAGAAGCTGTGCTACG |
| REV | TGTTGGCGTACAGGTCTTTG |
a
FOR, forward primer; REV, reverse primer; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.