TABLE 1.

ATR-IR data for the FMDV 2B synthetic peptides

Wave no. (cm−1)	Vibrationa	θ (°)b	Avg dichroic ratio ± SD	Avg S ± SDc	Avg γ┴ ± SDd	Avg γL ± SDe
Lipid alone
2,920	as CH2 stretching	90	1.94 ± 0.01	0.04 ± 0.01	53.19 ± 0.24
2,850	s CH2 stretching	90	1.75 ± 0.02	0.16 ± 0.01	48.49 ± 0.53
2,870	s CH3 stretching	0	3.97 ± 1.00	0.31 ± 0.10	42.44 ± 3.95
2B1
2,920	as CH2 stretching	90	1.53 ± 0.07	0.32 ± 0.05	42.41 ± 2.04
2,850	s CH2 stretching	90	1.40 ± 0.04	0.42 ± 0.04	38.24 ± 1.42
2870	s CH3 stretching	0	3.73 ± 0.33	0.33 ± 0.04	42.08 ± 1.63
1656	Amide I–α-helix	30	1.93 ± 0.04	−0.04 ± 0.02	56.22 ± 0.89	58.31 ± 2.12
2B2
2,920	as CH2 stretching	90	1.70 ± 0.03	0.19 ± 0.02	47.35 ± 0.68
2,850	s CH2 stretching	90	1.57 ± 0.02	0.28 ± 0.01	43.66 ± 0.53
2,870	s CH3 stretching	0	4.19 ± 0.72	0.36 ± 0.06	40.55 ± 2.57
1,656	Amide I–α-helix	30	2.44 ± 0.02	0.18 ± 0.01	47.62 ± 0.28	29.08 ± 1.99
2B3
2,920	as CH2 stretching	90	1.93 ± 0.08	0.04 ± 0.05	52.99 ± 2.02
2,850	s CH2 stretching	90	1.73 ± 0.04	0.17 ± 0.03	47.98 ± 1.07
2,870	s CH3 stretching	0	7.54 ± 4.34	0.40 ± 0.15	38.27 ± 6.52
1,630	Amide I–β-sheet	70	2.50 ± 0.21	−0.37 ± 0.13	90.00 ± 0.00	90.00 ± 0.00
2B4
2,920	as CH2 stretching	90	1.74 ± 0.03	0.17 ± 0.02	48.23 ± 0.72
2,850	s CH2 stretching	90	1.66 ± 0.01	0.22 ± 0.01	46.17 ± 0.37
2,870	s CH3 stretching	0	4.00 ± 0.61	0.34 ± 0.07	41.36 ± 2.73
1,656	Amide I–α-helix	30	2.05 ± 0.03	0.02 ± 0.01	53.84 ± 0.53	50.71 ± 2.45
2B4c
2,920	as CH2 stretching	90	1.77 ± 0.03	0.14 ± 0.02	49.09 ± 0.88
2,850	s CH2 stretching	90	1.66 ± 0.01	0.22 ± 0.01	46.10 ± 0.33
2,870	s CH3 stretching	0	4.30 ± 1.23	0.37 ± 0.13	40.24 ± 5.22
1,656	Amide I–α-helix	30	2.41 ± 0.02	0.17 ± 0.01	48.01 ± 0.34	22.42 ± 3.79

^aVibrations are presented as symmetric (s) or asymmetric (as).

^bθ, direction of the dipole moment associated with the vibration with respect to the direction of the main molecular axis (aliphatic chain or peptide-secondary structure).

^cS, form factor.

^dγ┴, angle between the direction of the molecular axis and the perpendicular to the crystal plane (similar to the membrane plane).

^eγL, angle between the direction of the peptide-secondary structure axis and the calculated aliphatic chain axis.