. 2018 Nov 12;92(23):e01360-18. doi: 10.1128/JVI.01360-18

TABLE 2.

Description of amino acid substitutions in the 2B mutant constructs

Location in 2B structure (residues)	Description of amino acid substitution	Name of construct	Name of peptide
Wild type	None	2B.WT

Helix 1 (60–78)	⁶²KLIK⁶⁵ → ⁶²AAAA⁶⁵	⁶²KLIK⁶⁵	2B2.1
	⁶⁸RLS⁷⁰ → ⁶⁸AAA⁷⁰	⁶⁸RLS⁷⁰	2B2.2
	⁶³K/⁶⁵K/⁶⁸R → ⁶³D/⁶⁵D/⁶⁸D	⁶³K/⁶⁵K/⁶⁸R	2B2.3

Connection (79–83)	⁷⁹RSKDP⁸³ → ⁷⁹AAAAA⁸³	⁷⁹RSKDP⁸³
	⁷⁹RS⁸⁰ → ⁷⁹AA⁸⁰	⁷⁹RS⁸⁰
	⁸³P → ⁸³A	⁸³P

Helix 2 (84–104)	⁸⁹ML⁹⁰ → ⁸⁹AA⁹⁰	⁸⁹ML⁹⁰
	⁹²DTG⁹⁴ → ⁹²AAA⁹⁴	⁹²DTG⁹⁴
	⁹⁹DSTF¹⁰² → ⁹⁹AAAA¹⁰²	⁹⁹DSTF¹⁰²
	⁹²N/⁹⁶Q/⁹⁹N → ⁹²Lys/⁹⁶Lys/⁹⁹Lys	⁹²N/⁹⁶Q/⁹⁹N

Helix 3 (121–141)	¹²⁸ILL¹³⁰ → ¹²⁸AAA¹³⁰	¹²⁸ILL¹³⁰	2B4.11
	¹³²GL¹³³ → ¹³²AA¹³³	¹³²GL¹³³	2B4.12
	¹³⁵ KVAS¹³⁸ → ¹³⁵AAAA¹³⁸	¹³⁵KVAS¹³⁸	2B4.13