FIG 3.
Acute norovirus infection is reduced in pIgR KO mice. Mice were infected by oral gavage with 3.8 × 105 PFU/animal neutral-red-labeled MNV-1, and tissues were harvested at 9, 18, and 48 hpi in a darkened room using a red photolight. The tissue homogenates were serially diluted and exposed to white light for 30 min. Replicated viral titers in the indicated tissues of WT and pIgR KO mice were assessed via plaque assay. The sensitivity threshold for each graph, indicated by the dotted line, is as follows (log PFU per gram of tissue): 2.17 (A), 2.21 (B), 2.3 (C), 2.37 (D), 2.27 (E), 2.22 (F), and 2.37 (G). The data are pooled from at least two independent experiments, and each symbol is a data point from an individual animal. The error bars represent SEM. The data were analyzed using the Mann-Whitney U test. *, P < 0.05; **, P < 0.01; ns, not statistically significant.