FIG 5.
Natural secretory immunoglobulins do not aid in MNV access to the Peyer’s patch. Intestinal ileal loop assays were performed in WT and pIgR KO mice. MNV-1 (100 μl; 3.8 × 105 PFU) was injected into the closed loop and incubated for 25 min. Viral genome copy equivalents (Equ.) were measured by qRT-PCR in Peyer’s patches. (A) MNV-1 bound to and was internalized in PP. The data were pooled from four independent experiments. (B) MNV-1 genomes bound to the follicle-associated epithelium (FAE Binding) and were internalized in the PP lamina propria (FAE Crossing). The data were pooled from four independent experiments. (C) MNV-1 was preincubated with rotavirus-specific sIgA or BSA for 1 h at 37°C before injecting the complex into the loop. The data are pooled from two independent experiments. Each symbol is a data point from an individual PP. The data mean is indicated for each group. The error bars represent SEM. The data were analyzed using the Mann-Whitney U test. ns, not statistically significant.