FIG 1.

Design of rIBV constructs. Schematic of wild-type BeauR and rIBV genomes generated by reverse genetics to display homologous spike genes in Beaudette backbone. The rIBVs generated expressed either the S1 and/or S2 ectodomain and transmembrane domain (TM) from M41 and QX wild-type virus; with M41 derived genes represented by red boxes and QX derived genes represented by green boxes. In all rIBVs the Beaudette backbone is represented by solid blue boxes, and the endodomain (E) of S2 from Beaudette is represented by shaded blue boxes. *, BeauR-M41(S) displays the full ectodomain of M41 spike, as previously described (22).