Viral load in the CALT and trachea in BeauR-M41(S)-, BeauR-M41(S1)-, and BeauR-M41(S2)-vaccinated chickens following challenge with M41-CK. (A to D) Relative viral RNA loads (expressed as corrected 40-CT) at 2 dpc (A and C) and 4 dpc (B and D). (E) Infectious viral load titers in trachea (4 dpc). Data points are shown as individual animals, and lines represent means ± SEM. Statistically significant differences between groups are highlighted. *, P < 0.05; **, P < 0.01; ***, P < 0.001.