TABLE 3.
Detection of IBV-derived RNA by RT-PCR in head-associated lymphoid tissues and tracheal samples following vaccination with BeauR-M41(S), BeauR-M41(S1), and BeauR-M41(S2)a
| Vaccination | dpv | No. of virus-positive tissues per group |
||
|---|---|---|---|---|
| Harderian gland | Trachea | Nasal turbinates | ||
| Mock | 1 | 0/5 | 0/5 | NA |
| BeauR-M41(S) | 1 | 3/5 | 0/5 | NA |
| BeauR-M41(S1) | 1 | 1/5 | 0/5 | NA |
| BeauR-M41(S2) | 1 | 1/5 | 2/5 | NA |
| Mock | 2 | 0/5 | 0/5 | 0/5 |
| BeauR-M41(S) | 2 | 1/5 | 0/5 | 3/5 |
| BeauR-M41(S1) | 2 | 0/5 | 0/5 | 3/5 |
| BeauR-M41(S2) | 2 | 0/5 | 0/5 | 2/5 |
| Mock | 4 | 0/5 | 0/5 | 0/5 |
| BeauR-M41(S) | 4 | 3/5 | 0/5 | 2/5 |
| BeauR-M41(S1) | 4 | 1/5 | 0/5 | 2/5 |
| BeauR-M41(S2) | 4 | 1/5 | 2/5 | 3/5 |
a
The results are depicted as the number of positive samples/number of birds per group (total of five birds/group). All positive results were confirmed by sequencing of PCR products (data not shown). NA, not applicable.