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. 2018 Nov 12;92(23):e01202-18. doi: 10.1128/JVI.01202-18

FIG 4.

FIG 4

Coimmunoprecipitation of NSs proteins with eGFP-IRF3. Selected 3×FLAG-tagged NSs proteins were coexpressed with eGFP-IRF3 in HEK293 cells. eGFP and ΔMx served as negative controls for eGFP-IRF3 and the NSs proteins, respectively. Cell lysates were subjected to immunoprecipitation via a GFP-binding nanobody immobilized on the bottom of a 96-well plate. Input samples and bound proteins were analyzed via immunoblotting (n = 3).