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. 2018 Nov 12;92(23):e01293-18. doi: 10.1128/JVI.01293-18

TABLE 1.

ASFV proteins identified by LC-MS/MSa

ORF Description/localization/function Mass No. of unique peptides
 Sequence coverage (%) Abundance (%) Previously detectede Reference(s)
S1 S2 S3
CP2475L Protein p150, polyprotein pp220-derived/core shell/morphogenesis 181.2 95 70 68 65.2 19.66 Y 14, 24, 32, 34
CP2475L Protein p34, polyprotein pp220-derived/core shell/morphogenesis 36.6 24 22 21 68.2 10.43 Y 14, 24, 32, 34
B646L Major capsid protein p72/capsid/morphogenesis 73.6 29 27 27 48.0 9.55 Y 27, 75
CP2475L Protein p37, polyprotein pp220-derived/core shell/morphogenesis 41.5 21 19 21 62.5 7.15 Y 14, 24, 32, 34
CP2475L Protein p14, polyprotein pp220-derived/core shell/morphogenesis 17.9 13 13 12 62.3 5.46 Y 14, 24, 32, 34
CP530R Protein p35, polyprotein pp62-derived/core shell/morphogenesis 35.2 18 18 18 73.8 5.04 Y 24, 33, 35
A104R Histone-like DNA-binding protein/nucleoid/morphogenesisd 11.6 5 7 6 69.2 4.64 Y 34, 39
B962L VACV I8-like RNA helicase/nucleoidd/transcriptiond 110.2 12 11 12 14.6 2.48 N 76
D1133L VACV D6-like RNA helicase/nucleoidd/transcriptiond 129.8 34 17 27 32.1 2.33 N 76
NP1450L RNA polymerase subunit 1/nucleoidd/transcriptiond 164.6 31 20 28 22.3 2.16 N 77
D117L Major transmembrane protein p17/inner envelope/morphogenesis 13.2 6 3 3 65.8 2.12 Y 31, 42
M1249L Uncharacterized protein 145.3 33 20 20 32.0 2.07 N
CP123L Uncharacterized protein/transmembrane domain 14.1 11 7 5 50.4 1.96 N
CP530R Protein p15, polyprotein pp62-derived/core shell/morphogenesis 17.9 8 8 6 45.6 1.67 Y 24, 33, 35
EP1242L RNA polymerase subunit 2/nucleoidd/transcriptiond 140.8 24 15 21 19.5 1.57 N 77
G1340L VACV A7 early transcription factor large subunit-like/nucleoidd/transcriptiond 155.8 23 12 21 19.8 1.39 N 4
C129R Uncharacterized protein 15.0 7 7 7 65.1 1.25 N
C717R Uncharacterized protein 84.1 14 13 13 18.1 1.12 N
A224L Inhibitor of apoptosis protein (IAP)/nucleoid/host defense 27.1 7 10 7 34.8 1.03 Y 60–62
I177L Uncharacterized protein; transmembrane domain 20.5 6 6 9 63.3 1.01 N
K145R Uncharacterized protein 17.2 1 3 10 63.4 0.95 N
B438L Protein p49/capsid/morphogenesis 49.6 10 10 10 23.3 0.93 Y 29
E248R Transmembrane myristoylated protein pE248R/inner envelope/entry 27.7 7 7 7 46.0 0.91 Y 10, 44
Q706L VACV D11-like helicase/nucleoidd/transcriptiond 80.9 16 9 8 22.4 0.85 N 78
K78R DNA-binding protein p10/nucleoid/morphogenesisd 8.4 3 2 3 39.7 0.85 Y 38
NP868R mRNA-capping enzyme/nucleoid/transcriptiond 100.4 19 7 9 21.3 0.84 Y 53
KP177R Membrane protein p22/inner envelope 20.7 7 5 7 46.9 0.79 Y 49
C475L Poly(A) polymerase/nucleoidd/transcriptiond 55.1 11 7 6 21.7 0.63 N 4
K421R Uncharacterized protein 49.7 6 7 8 17.8 0.58 N
E146L Uncharacterized protein; transmembrane domain 16.3 4 4 6 47.3 0.54 N
H359L RNA polymerase subunit 3-11/nucleoidd/transcriptiond 41.6 9 4 6 28.4 0.50 N 5
F317L Uncharacterized protein 36.8 5 6 6 22.7 0.48 N
R298L Serine/threonine-protein kinase 1 35.2 5 4 7 25.2 0.44 Y 66
CP530R Protein p8, polyprotein pp62-derived/core shelld/morphogenesisd 7.8 3 3 3 56.7 0.41 N 33
A137R Protein p11.5 16.2 1 4 5 31.4 0.39 Y 63
H240R Uncharacterized protein 27.7 6 4 4 27.9 0.38 N
CP312R Uncharacterized protein 35.2 2 2 8 33.3 0.35 N
E423R Uncharacterized protein 48.2 7 3 4 21.5 0.35 N
D205R RNA polymerase subunit 5/nucleoidd/transcriptiond 23.7 4 4 4 26.3 0.34 N 5
O174L DNA polymerase X/nucleoidd/DNA integrity 20.4 3 3 3 15.5 0.24 Y 57, 58
E199L Cysteine-rich transmembrane protein pE199L/inner enveloped/entryd 22.7 3 3 3 13.1 0.23 Y 41
NP419L DNA ligase/nucleoidd/DNA integrityd 48.5 3 2 5 8.8 0.23 N 56
E184L Uncharacterized protein 22.0 2 2 4 19.6 0.23 N
O61R Virus attachment membrane protein p12/inner envelope/entry 6.9 1 1 2 27.9 0.21 Y 40, 43
S273R Polyprotein processing protease/core shell/morphogenesis 32.0 2 3 3 12.1 0.20 Y 36, 37
D339L RNA polymerase subunit 7/nucleoidd/transcriptiond 39.1 2 3 3 11.2 0.20 N 5
C257L Uncharacterized protein; transmembrane domain 30.2 2 2 3 12.1 0.19 N
H171R Uncharacterized protein 19.9 2 2 3 19.3 0.18 N
EP152R Protein EP152R; transmembrane domain 18.0 1 1 3 19.1 0.17 N 79
B117L Uncharacterized protein; transmembrane domain 13.4 2 2 2 12.0 0.16 N
EP424R Putative RNA methyltransferase/nucleoidd/transcriptiond 49.6 2 1 4 10.8 0.15 N 4
B169L Uncharacterized protein; two transmembrane domains 18.8 2 2 2 16.6 0.15 N
H339R Alpha-NAC binding protein pH339R 40.0 1 1 4 10.6 0.14 N 65
CP204L Early phosphoprotein p32 23.6 2 1 3 11.8 0.13 Y 64, 80
E296R AP endonuclease/nucleoid/DNA integrity 33.9 2 2 2 6.4 0.13 N 55
E183L Transmembrane protein pE183L/inner envelope/morphogenesis 19.9 1 1 1 6.6 0.12 Y 30, 81
EP84R Uncharacterized protein; two transmembrane domains 9.0 1 1 1 23.8 0.12 N
C147L RNA polymerase subunit 6/nucleoidd/transcriptiond 16.8 2 1 2 15.0 0.11 N 82
QP383R Uncharacterized protein 42.9 2 2 1 7.0 0.11 N
E165R dUTPase/nucleoidd/DNA integrity 18.6 1 3 22.4 0.16 N 59
I73R Uncharacterized protein 8.5 1 2 26.0 0.14 N
B119L FAD-linked sulfhydryl oxidase 14.5 2 2 16.0 0.14 N 67, 68
C122R Uncharacterized protein 12.4 1 2 11.4 0.11 N
CP2475L Protein p5, polyprotein pp220 derived/core shell/morphogenesis 4.7 1 1b 43.2 0.11 N 32
E120R Protein pE120R/capsid/morphogenesis 13.6 1 1 9.2 0.08 Y 28
H124R Uncharacterized protein 15.0 1 1 4.8 0.08 N
EP402R Membrane glycoprotein similar to host CD2/outer envelope/host defense 46.5 1 1 2.5 0.06 Y 45–47
M448R Uncharacterized protein 52.4 1 1 2.0 0.05 N
H108Rc Transmembrane protein pH108R/inner envelope 12.5 1 13.9 0.05 Y 26
a

The data presented in Table 1 were compiled from three different ASFV samples (S1, S2, S3). ORFs present in all three samples (S1, S2, and S3) are in bold.

b

Protein p5 was detected in the global proteome of one virus stock and in the low-molecular-weight analysis of a second one.

c

Protein pH108R was detected in only one of the analyzed stocks but is included for reference as it was previously described as a structural component.

d

Proteins with a predicted function or with a subviral localization inferred from their known or predicted role.

e

Y, yes; N, no.