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. 2018 Nov 6;9:1293. doi: 10.3389/fphar.2018.01293

FIGURE 4.

FIGURE 4

Prevention of thrombin induced activation of PLCγ2 cascade by Fc. After incubating with different concentrations of Fc, WP was treated with thrombin for 3 min. (A) The phosphorylation of PLCγ2 and P47 in platelet were measured with western blotting. β-actin was used as a loading control. Platelet IP3 (B), DAG (C) and TXB2 (D) concentrations were assessed by corresponding ELISA kits. (E) Variation in [Ca2+] was assessed by detecting the fluorescence intensity emitted from intracellular Fluo-4. The images are from the peak point of each experiment. Tracings were from representative results in three independent experiments. Values are mean ± SEM (n = 6 for A; n = 4 for B,C; n = 3 for D). P < 0.05 versus control; #P < 0.05 versus thrombin only.