Figure 1.

Miniature inhibitory postsynaptic currents (mIPSCs) were recorded from individual inhibitory terminals. (A) GlyT2-eGFP⁺ neurons and neurites were visible in our spinal cord cultures. eGFP⁺ neurites with a parallel structure and beaded varicosities (*) were labeled with FM 4–64 dye (B), confirming they are glycine-containing presynaptic terminals. Scale bars are 10 μm. (C) Cartoon of the paired recording configuration that allowed identification of mIPSCs that originated from an individual inhibitory terminal (top). Postsynaptic mIPSCs were simultaneously recorded with the whole-cell patch clamp pipette (WC) and the loose-patch clamped pipette (LP). The traces (middle and bottom) are a continuous 2 s recording from one WC-LP pair. (D) The LP and whole cell mIPSCs from the same recording as displayed in (C), were strongly correlated in time and amplitude, as expected from an extracellular and intracellular measurement of the same signal.