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. 2018 Nov 6;9:1624. doi: 10.3389/fpls.2018.01624

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Copyright © 2018 Meng, Zhang, Yan, Lin, Wang, Huang, Huang, Li, Yu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of chromosomal rearrangement between sorghum and S. spontaneum by oligo-FISH. (A,B) A FISH assay of oligo probes Sb2 (green) and Sb8 (red) in sorghum BTx623 and S. spontaneum SES208. The FISH result confirmed that probes Sb2 and Sb8 are located on different chromosomes in sorghum (A) but are located at the opposite ends of the same chromosome in S. spontaneum SES208 (B). (C,D) A FISH assay of oligo probes Sb5 (red) and Sb7 (green) in sorghum BTx623 and S. spontaneum SES208. The FISH result revealed that probes Sb5 and Sb7 are located on different chromosomes in sorghum (C) but are located at the opposite ends of the same chromosome in S. spontaneum SES208 (D). The arrows indicate the chromosomes bearing FISH signals. Scale bars, 10 μm.