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. 2018 Nov 6;9:1624. doi: 10.3389/fpls.2018.01624

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Copyright © 2018 Meng, Zhang, Yan, Lin, Wang, Huang, Huang, Li, Yu, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Further validation of chromosomal rearrangements between sorghum and S. spontaneum by oligo-FISH. (A,B) A FISH assay of oligo probes Sb5.1 (green) and Sb6 (red) in sorghum BTx623 (A) and S. spontaneum SES208 (B). The FISH result confirmed that the probes Sb5.1 and Sb6 are located on different chromosomes in sorghum (A) but in the opposite ends of the same chromosome in S. spontaneum SES208 (B). (C,D) A FISH assay of oligo probes Sb8.1 (green) and Sb9 (red) in sorghum BTx623 (C) and S. spontaneum SES208 (D). The FISH result confirmed that probes Sb8.1 and Sb9 are located on different chromosomes in sorghum (C) but on the same chromosome in S. spontaneum SES208 (D). Arrows indicate the chromosomes bearing FISH signals. Scale bars, 10 μm.