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. 2018 Nov 6;12:398. doi: 10.3389/fncel.2018.00398

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Copyright © 2018 García-Domínguez, Veselá, García-Revilla, Carrillo-Jiménez, Roca-Ceballos, Santiago, de Pablos and Venero.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipopolysaccharide/MPTP induce the appearance of galectin-3 (Gal3)-expressing microglial cells in SN. Immunofluorescence for Iba1 (A,D,G,J,M) and Gal3 (B,E,H,K,N) in the different treatment assayed. Only LPS/MPTP group shows a clear expression of Gal3 microglial cells (merged images, C,F,I,L,O). Scale bar: 100 μm. (M–O) Shows high magnification pictures of Iba-1, Gal3 and merge image of the LPS/MPTP group. Scale bar: 500 μm. (P) Quantification of Iba-1/Gal3 co-localizing cells in the different treatment assayed. Results are mean ± SD of at least three independent experiments and are expressed as number of cells. One-way ANOVA followed by the Fischer’s LSD post hoc test for multiple comparisons was used for statistical analysis, with α = 0.05: ^∗, compared with the control; p < 0.05.