Table 3.
ARNm target | Primers sense (S) and antisense (A) sequences | Temp (°C) |
---|---|---|
β-Actin | S:5′- GGCTATGCTCTCCCTCACG A:5′- CTTCTCTTTGATGTCACGCACG |
60 |
TNF-α | S:5′- TGCCTATGTCTCAGCCTCTTC A: 5′- GAGGCCATTTGGGAACTTCT |
60 |
INF-β | S:5′- AACTTCCAAAACTGAAGACC A:5′- AACTCTGTTTTCCTTTGACC |
60 |
IL-6 | S:5′- GACAAAGCCAGAGTCCTTCAGA A:5′- AGGAGAGCAATTGGAAATTGGGG |
60 |
IL-1β | S:5′- TGTAATGAAAGACGGCACACC A:5′- TCTTCTTTGGGTATTGCTTGG |
60 |