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. 2018 Nov 6;12:398. doi: 10.3389/fncel.2018.00398

Table 3.

Primers used for real-time PCR.

ARNm target Primers sense (S) and antisense (A) sequences Temp (°C)
β-Actin S:5′- GGCTATGCTCTCCCTCACG
A:5′- CTTCTCTTTGATGTCACGCACG
60
TNF-α S:5′- TGCCTATGTCTCAGCCTCTTC
A: 5′- GAGGCCATTTGGGAACTTCT
60
INF-β S:5′- AACTTCCAAAACTGAAGACC
A:5′- AACTCTGTTTTCCTTTGACC
60
IL-6 S:5′- GACAAAGCCAGAGTCCTTCAGA
A:5′- AGGAGAGCAATTGGAAATTGGGG
60
IL-1β S:5′- TGTAATGAAAGACGGCACACC
A:5′- TCTTCTTTGGGTATTGCTTGG
60