Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec;121:38–49. doi: 10.1016/j.neuint.2018.10.014

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Fig. 4 — GAP-43 mRNA and protein expression during hiPSC differentiation to cortical neurons. (A) Expression of GAP-43 mRNA was detected by quantitative PCR; GAP-43 mRNA was first expressed on d18 of differentiation. A marked increase in GAP-43 mRNA expression was seen between d18 and d60. On d60, GAP-43 mRNA was expressed the highest; thereafter the levels decreased and plateaued until d165 of differentiation. After d165, there was a trend towards decreased expression of GAP-43. GAP-43 mRNA was normalized to the average of HPRT-1, RPL-27 and RPL-30 mRNA. GAP-43 mRNA normalized change at each differentiation time point was related to the highest GAP-43 mRNA expression, which was set to 1. For a summary of assigned significances for the measured changes of GAP-43 mRNA, see Supplementary Table 4. (B) GAP-43 protein levels were detected by western blot and GAP-43 protein was first detected on d18. GAP-43 levels increased gradually between d18 and d60, when it was expressed the highest. Thereafter, GAP-43 levels slightly decreased and plateaued until d165, which was followed by a gradual decrease in GAP-43 levels. GAP-43 levels were correlated to GAPDH and GAP-43 fold change in each differentiation was related to the highest GAP-43 protein level, set to 1. Representative bands of GAP-43 and GAPDH are shown. The summary of assigned significances for the measured changes of GAP-43 protein is shown in Supplementary Table 5. (C) Total GAP-43 secretion was detected by ELISA; GAP-43 was first observed to be secreted on d18. Between d18 and d45, secreted GAP-43 levels plateaued; thereafter there was a trend towards gradual increase of secreted GAP-43 between d60 and d120. On d120, GAP-43 was secreted the highest followed by a gradual decrease in GAP-43 secretion. The summary of assigned significances for the changes of secreted GAP-43 concentrations is summarized in Supplementary Table 6. Bars represents mean±SEM. For intracellular expression of GAP-43 mRNA and protein, n = 4 (except from d165 to d210 where n = 2) and for secreted GAP-43, n = 5 (except from d165 to d210 where n = 3).