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Translation efficiency of polyadenylated RNAs encoding firefly luciferase and capped with 1–5 analogues, or reference caps, in mammalian cells. Electroporation was performed with 2.5 µg of each RNA in Huh-7 cells and luciferase expression was measured at time points specified on the top. Prior to transfection, RNA quality was validated by agarose electrophoresis (left panel). Data from duplicate experiments (two measurements per sample) are depicted ±s.d. (Online version in colour.)
