Figure 7.

Study of protein–RNA interactions by UV-cross-linking and afinity capture of single-biotin-capped RNA. Schematic of RNA-binding protein recovery using biotinylated 3′NTR of HCV (a) capped with analogue 3. The interaction of eGFP (b) and hnRNPC-eGFP (c) with the biotinylated RNA was evaluated by measuring the protein recovery upon RNA affinity capture using western blot and eGFP-specific antibodies. Standards of known amount (on the left of the panels) were used to calculate the percentage of protein recovery (numbers below). Panels on the right are longer exposures of the panels on the left. Protein markers (kDa) are indicated on the right side of each panel. For further details see text. (Online version in colour.)